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Sterilisation & Asepsis



Process where by all living organisms including spores are destroyed


Substances which kill potentially infectious organisms, and are usually used on surfaces (not the skin)
Destroys only vegetative forms of the organisms


Preventing wound contamination by ensuring that only sterile material makes contact with the wound and that airborne transmission of organisms is minimised


(Mild disinfectants) Relatively non-toxic non-irritant anti microbial agents -> can be applied topically to the body
Iodine best skin preparation but irritant to raw areas (Betadine) Must remain on surface for 60 sec -> lead to effect
Alcohol pure solution useless as a disinfectant but 50 - 70% solution useful skin antiseptic
Chlorhexidine (phenolic derivative) powerful antiseptic agent against vegetative organisms -> good skin antiseptic with low toxicity

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